Lpme is a technique for sample preparation that is extremely simple, affordable and virtually a solventfree. Hollow fibre liquidphase microextraction principles. Recent developments in solid phase microextraction. An enrichment factor upto 28,050 times was obtained that is higher than already published results. Liquid phase microextraction combined with hollow fiber as a sample preparation technique prior to gas chromatographymass spectrometry. Such systems are usually referred to as liquid phase microextraction lpme systems either 2 phase or 3 phase lpme. Hollow fiber liquid phase microextraction hflpme offers an efficient alternative to classical techniques for sample preparation and preconcentration. In the present study, hollow fiber liquidphase microextraction hflpme method was used to preconcentrate trace amount of phenol prior to its spectrophotometric determination. Both methodologies, that is, static lpme with hf and dynamic lpme with hf, involved the use of a small volume of organic solvent impregnated in the hollow fiber, which was held by the needle of a.
Initial experiments verified that this technique is applicable on aqueous as well as suspensions of solid samples, thus. Trace determination of tamoxifen in biological fluids. The new metal alloy design includes a thicker, flexible plunger that is much less likely to kink or break, and helps to reinforce the needle especially when used in an autosampler with a. A sampling chamber was prepared for sampling of ma with hfsupported deionized water absorbency. This thesis addresses the application of hollow fiber liquid phase microextraction as a simple, efficient and environmentally friendly tool to study the fate of four common antiinflammatory drugs ketoprofen, naproxen, diclofenac and ibuprofen during wastewater treatment. A general equilibrium equation can be written describing. A novel application of three phase hollow fiber based liquid phase microextraction hflpme for the hplc determination of two endocrine disrupting compounds edcs, noctylphenol and nnonylphenol, in environmental waters. Solid phase microextraction spme samantha keene tuesday, 30 june 2009 who discovered spme. The desorbed analytes were analyzed using reversedphase high performance liquid. The analysis of plant growth regulators presents a challenge due to their trace quantities and complex matrices. Hollow fiber liquid phase microextraction hflpme in contrast with other techniques, hflpme is again quite simple, but it requires additional support and some automation to be successful.
Using hollow fibres, it is possible to perform extractions for sample cleanup and enrichment with very cheap and simple equipment, leading to high enrichment factors easily thousands of times for ultra trace analysis. A free powerpoint ppt presentation displayed as a flash slide show on id. A new hollow fiber liquid solid phase microextraction for extraction of ginsenosides from a chinese compound formula was established using al 2 o 3 nanoparticles as the adsorbent. Typically, the porous hollowfiber membrane is first impregnated and filled with a few microliters of organic solvent, inside the lumen of the membrane, in the socalled twophase hflpme. Into of lumen of 30 mm of accurel membrane 3001200 pp membrana gmbh wuppertalgermany was put a 10 mm metallic piece, functionalizing the hollow fiber as a solvent bar and was used for.
In this method, the aerosol samples were dissolved in 0. Development of a new liquid phase microextraction method with. Read comparison between solid phase microextraction spme and hollow fiber liquid phase microextraction hflpme for determination of extractables from postconsumer recycled pet into food simulants, talanta on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. By continuing to use our website, you are agreeing to our use of cookies. This research develops a rapid method for sampling and analysis of maleic anhydride ma in air using a onestep hollow fiber hf membrane in the liquid phase followed by highperformance liquid chromatography. Hollow fiberliquid phase microextraction hflpme has several advantages over the other membrane techniques used for. Recent developments in solid phase microextraction techniques oppiaine laroamne subject analytical chemistry tyon lajiarbetets art level m. Phenol reacted with 4aminoantipyrine 4aap reagent in presence of potassium hexacyanoferrate iii and then was extracted into the octanol extractant inserted into the lumen and pores of hollow fibers. Spectrophotometric determination of trace phenol in. Lpme method was established for purification and enrichment of glutathione gsh in human saliva followed by a miniaturized capillary electrophoresis with amperometric detection system mini. Equilibrium sampling of freely dissolved alkylphenols into a.
Pdf hollow fiber liquid phase microextraction followed. The lumen of a polypropylene hollow fiber is filled with aqueous acceptor solution ph 9. Development of membrane hollow fiber for determination of. Vortexassisted hollowfiber liquidphase microextraction coupled. Hollow fiber liquid phase microextraction followed by high performance liquid chromatography for determination of ultratrace levels of seiv after derivatization in urine, plasma and natural water samples. It can provide a high degree of selectivity and enrichment by eliminating carryover between single runs. Hflpme was applied for the first time to aerosols for pinic and pinonic acid analysis.
Sorption and transformation of polar pharmaceuticals during. Spme is amenable to automation using an autosampler. Pdf hollow fiber liquidphase microextraction hflpme offers an efficient alternative to. Development of hollowfiber liquidphase microextraction. School of pharmacy, university of oslo, oslo, norway.
Equilibrium sampling of freely dissolved alkylphenols into. Seidi s, yamini y, rezazadeh m, electrically enhanced microextraction for. Development of hollowfiber liquidphase microextraction method. Application of hollow fiber assisted liquid phase microextraction to identify avermectins in stream water using msms. A variety of solvents are known for the extraction of the various. If so, let me know the supplier of the fibers, the compounds you have been extracting and your perception of the method. Hollow fiber based liquid phase microextraction combined.
Application of coated hollow fiber protected membrane liquid. A new hollow fiber liquidsolid phase microextraction using. For hflpme 1heptanol, 2octanone and dibutylether were studied as extracting solvents. Ultrasoundassisted hollow fibre liquidliquidliquid. A simple and highly sensitive method that involves threephase hollow fiber supported liquidphase microextraction hflpme coupled with maltodextrin mdmodified capillary electrophoresis ce was successfully developed for the separation and determination of trace concentrations of citalopram cit enantiomers in urine samples. A simple and highly sensitive method that involves three phase hollow fiber supported liquid phase microextraction hflpme coupled with maltodextrin mdmodified capillary electrophoresis ce was successfully developed for the separation and determination of trace concentrations of citalopram cit enant. To decrease the amount of manual work, promising steps toward automation were taken.
Determination of metal ions by ultrasound assisted hollow. Read miniaturized hollow fiber assisted liquidphase microextraction with in situ derivatization and gas chromatographymass spectrometry for analysis of bisphenol a in human urine sample, journal of chromatography b on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Pdf on jan 1, 20, astrid gjelstad and others published perspective. Application of dynamic liquidphase microextraction to the. These methods have various drawbacks, including high cost and excessive preparation time. Previous work on dynamic lpme11,12 was based on manual manipulation of the syringe plunger, a tedious operation. Hollow fiber headspace liquidphase microextraction hfhslpme method. Hollow fiber liquid phase microextraction hflpme three phase aqueousorganicaqueous extraction of nsaids from water or sludge slurry samples the lumen of a polypropylene hollow fiber is filled with aqueous acceptor solution ph 9. Sorption and transformation of polar pharmaceuticals. Jul 01, 2008 read miniaturized hollow fiber assisted liquidphase microextraction with in situ derivatization and gas chromatographymass spectrometry for analysis of bisphenol a in human urine sample, journal of chromatography b on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Abstract a solid bar microextraction sbme method containing sorbent materials 2 mg in the lumen of a porous hollow fiber membrane 2. In general, most hflpme reports performed using stirring to help mass transfer were very slow 1921. Pdf application of hollowfiberassisted liquidphase. Hollowfiber solidphase microextraction hf spme, in spite of using a.
Hollow fiber liquidphase microextraction hflpme offers an efficient alternative to classical techniques for sample preparation and preconcentration. In recent years, hollowfiber liquidphase microextraction hflpme, a new miniaturized format of lle, has been developed for separation and. Two modes of liquidphase microextraction lpme combined with hollow fiber hf were developed for gas chromatographymass spectrometry gcms. Liquidphase microextraction combined with hollow fiber as. Solid phase microextraction solid phase microextraction solid phase microextraction spme is a technique for extracting analytes from solid, liquid or gaseous samples by adsorbing them onto the spme fiber and then desorbing them into an inlet, either on a gas chromatograph gc or an hplc system. Another solventminimized microextraction technique known as hollow fiber liquidphase microextraction hflpme 23, 24, has also been reported for the analysis of selected drugs in urine samples. Comparison between solid phase microextraction spme and. Hflpme is simple, low cost, uses minimum solvent l and provides a high level of enrichment 25. That means that glyphosate can be extracted from a very dilute solution using the socalled hollow. Dcf was derivatized and extracted simultaneously using vortexassisted liquidliquid microextraction vallme prior to.
Hollow fiberliquid phase microextraction for trace enrichment of the. Hollow fiber liquid phase microextraction hflpme and solid phase microextraction spme methods for preconcentration of contaminants toluene, benzophenone, tetracosane and chloroform in food simulants were investigated. Multiwalled carbon nanotubes as efficient sorbent for the. Ghambarian m, yamini y, esrafili a, three phase hollow fiber liquid phase microextraction based on two immiscible organic solvents for determination of tramadol in urine and plasma samples journal of pharmaceutical and biomedical analysis 56 5. The enhancement technique involved dispersing al 2 o 3 particles in nbutanol to fill the pores and lumen. Hollow fiber based liquid phase microextraction combined high. For the first time, hollowfiber liquidphase microextraction combined with highperformance liquid chromatographyultraviolet was used to extract trans,transmuconic acid, in urine samples of workers who had been exposed to benzene. This study presents a hollow fiberbased liquid phase microextraction hflpme method combined with an hplc dadfld determination using a highly packed chromatographic column that allows a simple, lowcost, accurate, high sensitive and selective methodology for the determination of eight widely used fluoroquinolones.
Determination of ketamine, norketamine and dehydronorketamine. A simple and highly sensitive method that involves threephase hollow fiber supported liquidphase microextraction hflpme coupled with maltodextrin md modified capillary electrophoresis ce was successfully developed for the separation and determination of trace concentrations of citalopram cit enantiomers in urine samples. Application of hollow fiber liquid phase microextraction. Such systems are usually referred to as liquid phase microextraction lpme systems either 2phase or 3phase lpme. Development of a new liquid phase microextraction method. Application of hollow fiber liquid phase microextraction for. Hollow fiber protected liquid phase microextraction of triazine herbicides. Recent developments in solid phase microextraction techniques.
Jan 14, 2014 liquid phase microextraction lpme enables analytes to be extracted with a few microliters of an organic solvent. A new hollow fiberliquidsolid phase microextraction for extraction of ginsenosides from a chinese compound formula was established using al2o3 nanoparticles as the adsorbent. A novel liquid phase microextraction lpme method has been developed, by coating with hollow fiber a metallic paper pin. Hollow fiberbased liquid phase microextraction hflpme. Solidphase microextraction 235 on gasliquid or liquidliquid partitioning. The parameters affecting the metabolite extraction were optimized as follows. Ionic liquidbased hollowfiber liquidliquidliquid microextraction ilhflllme coupled to capillary electrophoresis ce has been developed for t we use cookies to enhance your experience on our website. A new hollow fiber liquid solid phase microextraction for extraction of ginsenosides from a chinese compound formula was established using al2o3 nanoparticles as the adsorbent. The spme metal alloy fiber assemblies are manufactured with a flexible metal alloy used in the needle, plunger, and fiber core.
Microscale liquidliquidliquid extraction in the 96well format. The enhancement technique involved dispersing al2o3 particles in nbutanol. Different factors that can affect the extraction process such as extraction solvent, acceptor and donor phase composition, salt addition, stirring rate, extraction. Hollow fiberprotected liquidphase microextraction of triazine herbicides. Liquid phase microextraction lpme enables analytes to be extracted with a few microliters of an organic solvent. Sep 01, 2014 read comparison between solid phase microextraction spme and hollow fiber liquid phase microextraction hflpme for determination of extractables from postconsumer recycled pet into food simulants, talanta on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. A threestage thermoelectric cooler was used for cooling a copper rod coated with a polydimethylsiloxane pdms hollow fiber, which served as the spme fiber. A method based on hollow fiber liquid phase microextraction hflpme for analysis of pinic acid and pinonic acid was developed and for the first time successfully applied to ambient aerosol samples. Introduction the big demand of food leads to use different kind of pesticides in order to protect the crops and increase their. Analysis of diclofenac in water samples using in situ. The enhancement technique involved dispersing al2o3 particles in nbutanol to fill the pores and lumen of a polyether sulfone membra.
The applicability of hollow fiber liquid phase microextraction hflpme combined with highperformance liquid chromatographyultraviolet detection hplcuv was evaluated for the extraction and determination of tamoxifen tam in biological fluids including human urine and plasma. Three phase aqueousorganicaqueous extraction of nsaids from water or sludge slurry samples. Pdf developments in hollow fiber based liquidphase. Hollow fiber supported liquidphase microextraction. In the present study, hollow fiber liquid phase microextraction hflpme method was used to preconcentrate trace amount of phenol prior to its spectrophotometric determination. The application of microextraction for the determination of drugs in biological samples 3 febri annuryanti 10145222 liquid phase microextraction lpme 2. A variety of solvents are known for the extraction of the. A new hollow fiber liquidsolid phase microextraction. Liquidphase microextraction combined with hollow fiber as a. A novel, simple, and effective analytical method for the determination of three trace.
In this study, a vortexassisted threephase hollow fiber liquidphase microextraction vahflpme coupling with high performance liquid chromatography. Determination of metal ions by ultrasoundassisted hollow fiber liquidphase microextraction technique. A new analytical method by graphenereinforced hollow fiber liquid phase microextraction combined with highperformance liquid chromatography fluorescence detection was developed for the determination of bisphenol a and 4tertbutylphenol in bottled juices. Application of coated hollow fiber protected membrane. Applications of hollowfiber and related microextraction. Liquidphase microextraction combined with hollow fiber as a sample preparation technique prior to gas chromatographymass spectrometry. Another solventminimized microextraction technique known as hollow fiber liquid phase microextraction hflpme 23, 24, has also been reported for the analysis of selected drugs in urine samples. Ghambarian m, yamini y, esrafili a, threephase hollow fiber liquidphase microextraction based on two immiscible organic solvents for determination of tramadol in urine and plasma samples journal of pharmaceutical and biomedical analysis 56 5. Liquid phase microextraction ali sarafrazyazdi, amirhassan amiri the development of faster, simpler, inexpensive and more environmentallyfriendly samplepreparation techniques is an impo. Here, we present a method for the determination of ketamine kt and its main metabolites, norketamine nk and dehydronorketamine dhnk in urine samples by using hollowfiber liquidphase microextraction hflpme in the threephase mode. Hollow fiber supported liquidphase microextraction combined. Is anyone using hflpme, hollow fiber liquid phase microextraction. Hplpme relies on the extraction of target analytes from aqueous samples into a supported liquid membrane slm sustained in the pores of. Efficiency of hollow fiber liquidphase microextraction.
Solid phase microextraction fiber assemblies supelco bellefonte, pa t794123m 1999 sigmaaldrich co. Determination of melamine in soil samples using surfactant. Bioanalytical applications of solid phase microextraction coupled to lcms heather lord, marcel musteata, dajana vuckovic, simon zhou and janusz pawliszyn department of chemistry. A cold fiber solid phase microextraction device was designed and constructed based on the use of a thermoelectric cooler tec. Hollow fiberbased liquid phase microextraction hflpme as. Spme to be automated compared to the manual operation of hflpme makes.
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